“[GaboScope] Numerically enhanced lensless Gabor microscopy for high-throughput marker-free investigation of dynamic live biosamples” (acronym “GaboScope”)

funding Source: National Science Centre, Poland (Sonata)
team: Dr. Eng. Julianna Winnik (PW), Dr. Eng. Marcin Marzejon (PW), Dr. Eng. Angika Bulbul (PW), M.Sc. Eng. Piotr Arcab (SD PW), M.Sc. Eng. Emilia Wdowiak (SD PW), M.Sc. Eng. Bartosz Mirecki (SD PW), Dr. Eng. Mikołaj Krysa (PW)

partners:

• Dr. hab. Marzena Stefaniuk’s group from the Nencki Institute of Experimental Biology, PAS
• Dr. Luiza Stanaszek’s group from the Institute of Experimental and Clinical Medicine, PAS
• Prof. Vicente Micó’s group from the University of Valencia, Spain
• Prof. Chao Zuo’s Smart Computational Imaging Lab from Nanjing University of Science and Technology, China

project Description:
The GaboScope project focuses on advancing Gabor holographic microscopy for rapid, lensless imaging of live cells in large measurement volumes without the use of fluorescent markers. The advantages of this technique include a compact system design, on-axis hologram acquisition, and an extended depth of field, which together facilitate imaging of large volumes. However, the method faces challenges such as low resolution due to limited magnification and the twin-image problem. In this project, we plan to develop advanced numerical algorithms that account for beam sphericity and multiple scattering, as well as noise reduction techniques and automatic focal plane detection. Our solutions will support new lensless systems, such as those employing wavelength multiplexing illumination or dual quasi-point light sources. Planned biomedical applications include the investigation of live cells (e.g., sperm and neurons) and tissue fragments (e.g., mouse brain tissue).