„Lensless holographic microscope enabling label-free high-throughput biomedical sample examination” (acronym „µHOLLO”)

funding agency: The National Centre for Research and Development (Lider)

team: dr inż. Julianna Winnik (PW), dr inż. Mikołaj Rogalski (PW), dr inż. Paweł Matryba (Nencki PAN), mgr inż. Emilia Wdowiak (SD PW)

partners: Nencki Institute for Experimental Biology PAS, Life Institute

project description:

Project contract no. LIDER14/0329/2023, funded by the National Centre for Research and Development under the LIDER XIV programme. The aim of the μHOLLO project is to develop a novel product — a lensless holographic microscope (LHM) that enables rapid and non-invasive analysis of large measurement volumes for biomedical applications, such as the histopathological analysis of tissue fragments and cell phenotyping and counting without the need for staining (a costly and time-consuming process detrimental to live samples). The LHM, by not employing a microscope objective, is fortunately free from the limitations of field of view, depth of focus, and aberrations associated with imaging optical elements. It is based on Gabor holography, which functions well for weak light-scattering objects but encounters significant challenges when analyzing thicker samples that carry crucial three-dimensional information about the examined object. The innovation of the planned new product lies in the application of illumination with an optimized degree of spatio-temporal coherence and wavelength, along with novel holographic reconstruction algorithms specialized for biological objects.

link: https://qcilab.mchtr.pw.edu.pl/